Stanford University PhD Dissertation Defense
Interactions between lipid bilayers and inorganic material surfaces
Morgan Mager
Research Advisor: Nicholas Melosh
Department of Materials Science and Engineering
Thursday, April 9, 2009 @ 1:00 p.m. (refreshments served at 12:30 p.m.)
Location: CISX Auditorium
Abstract:
Because of their unique biological and material properties, lipid bilayers have been extensively studied for use in biosensor and drug delivery applications. In the past, these systems have mostly taken the form of bulk solutions. More recently, researchers have integrated bilayers with chip-based architectures to take advantage of advanced optical, scanning probe and electronic characterization. These applications typically involve the creation of hybrid devices with inorganic and bilayer components, both of which affect the final device performance. In particular, the properties of supported lipid bilayers (SLBs) are known to depend on the substrate chemistry and topography as well as the lipid used. In spite of the large body of work involving these systems, there is still much that remains unknown about the formation and ultimate structure of the interface between these very different materials. One outstanding question in the study of SLBs is the role that the bilayer deposition method plays in determining the bilayer properties. In the work I will present, we have developed a new method for forming and patterning lipid bilayers: bubble collapse deposition (BCD). This method is similar to an in situ version of Langmuir-Blodgett deposition, and offers unique possibilities for the fabrication of lipid-based devices. With BCD, we have demonstrated the first SLB formation on alumina using uncharged lipids. We have also used BCD to produce a hybrid lipid-gated chemical delivery device, and to directly transfer lipids from a cell membrane to a substrate surface. I will discuss these applications and the fundamental insights they offer into the interactions between lipids and common semiconductor fabrication materials.
Interactions between lipid bilayers and inorganic material surfaces
Morgan Mager
Research Advisor: Nicholas Melosh
Department of Materials Science and Engineering
Thursday, April 9, 2009 @ 1:00 p.m. (refreshments served at 12:30 p.m.)
Location: CISX Auditorium
Abstract:
Because of their unique biological and material properties, lipid bilayers have been extensively studied for use in biosensor and drug delivery applications. In the past, these systems have mostly taken the form of bulk solutions. More recently, researchers have integrated bilayers with chip-based architectures to take advantage of advanced optical, scanning probe and electronic characterization. These applications typically involve the creation of hybrid devices with inorganic and bilayer components, both of which affect the final device performance. In particular, the properties of supported lipid bilayers (SLBs) are known to depend on the substrate chemistry and topography as well as the lipid used. In spite of the large body of work involving these systems, there is still much that remains unknown about the formation and ultimate structure of the interface between these very different materials. One outstanding question in the study of SLBs is the role that the bilayer deposition method plays in determining the bilayer properties. In the work I will present, we have developed a new method for forming and patterning lipid bilayers: bubble collapse deposition (BCD). This method is similar to an in situ version of Langmuir-Blodgett deposition, and offers unique possibilities for the fabrication of lipid-based devices. With BCD, we have demonstrated the first SLB formation on alumina using uncharged lipids. We have also used BCD to produce a hybrid lipid-gated chemical delivery device, and to directly transfer lipids from a cell membrane to a substrate surface. I will discuss these applications and the fundamental insights they offer into the interactions between lipids and common semiconductor fabrication materials.
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