Please join the Stanford Optical Society for the following seminar presented by Prof. Bo Huang of the University of California, San Francisco. Refreshments will be served at 4:00 PM in the Applied Physics building lobby.
Special Seminar
Bo Huang
University of California, San Francisco
STORM: Super-Resolution Light Microscopy with Twinkling Molecules
Thursday, June 10, 4:15 PM, AP 200. Refreshments at 4:00
Presented by the Stanford Optical Society
Abstract
The ability of fluorescence microscopy to perform noninvasive imaging of live samples with molecular specificity has made it one of the most powerful imaging techniques to study cellular processes. However, the diffraction of light limits the spatial resolution of conventional fluorescence microscopy, leaving many biological structures too small to be observed in detail. To overcome this limit, we have developed the Stochastic Optical Reconstruction Microscopy (STORM) technique. It utilizes the photoswitching of fluorophores to isolate their spatially overlapped images, and single-molecule localization to reconstruct the sample structure with the position of labeled fluorescent probes. We have achieved a 20-30 nm lateral resolution in cellular samples, which is an improvement by more than an order of magnitude over conventional fluorescence microscopy. The incorporation of three-dimensional (3D) single molecule localization further enables 3D STORM of a whole cell with 50-60 nm axial resolution. We have also created photoswitchable fluorophores for multicolor imaging by combinatorial pairing of various activator dyes and reporter dyes. We have demonstrated the ability of STORM to visualize structures unresolvable by conventional fluorescence microscopy, including in vitro reconstituted clathrin-mediated endocytic machinery and synapses in the olfactory system.
About our speaker
Dr. Bo Huang received his B.S. degree in Chemistry from Peking University in China in 2001. In 2006, he earned his Ph. D. degree in Chemistry at Stanford University under the direction of Dr. Richard N. Zare. After working as a postdoc in Dr. Xiaowei Zhuang’s lab at Harvard University, he joined the faculty of the University of California, San Francisco in 2009 as an Assistant Professor of Pharmaceutical Chemistry and Biochemistry& Biophysics. Dr. Huang’s research work encompasses the area of bioanalysis, single molecule biophysics and optical microscopy. As a postdoc, he and his colleagues developed the super-resolution microscopy technique of STORM. He is currently interested in using optical methods to probe biological processes at the molecular scale. The awards that Dr. Huang has received include the Stanford Graduate Fellowship, the GE Healthcare and Science Prize for Young Life Scientists, and the Searle Scholar.
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